Thermo Fisher Scientific HEF1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence:
  MKYKNLMARA LYDNVPECAE ELAFRKGDIL TVIEQNTGGL EGWWLCSLHG RQGIVPGNRV KLLIGPMQET ASSHEQPASG LMQQTFGQQK LYQVPNPQAA PRDTIYQVPP SYQNQGIYQV PTGHGTQEQE VYQVPPSVQR SIGGTSGPHV GKKV

• Positive Samples: B-cell

• Cellular Location: Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Nucleus, Cell cortex, Cytoskeleton, Focal adhesion, Lamellipodium, Spindle

Target Information

HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis, and oncogenic transformation. The Cas family of docking proteins, including HEF1, are substrates of focal adhesion kinase (FAK) and Src family tyrosine kinases, which are active targets for drug development.
Overexpression of HEF1 increases mRNA levels of proteins related to motility and invasiveness, such as metalloproteinases, MLCK, p160ROCK, and ErbB1. HEF1 also mediates apoptosis in epithelial-derived cell lines (e.g., MCF7, HeLa).
Clinical studies have shown that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance, emphasizing the importance of Cas family proteins in cancer prognosis.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information: www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.