Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (rVU-1D9)
Ep-CAM/CD326 단백질을 표적하는 인간 특이 마우스 단클론 항체로, IHC(P), IHC(PFA), Flow Cytometry 등에 사용 가능. Ep-CAM은 상피세포 접착 및 증식 관련 마커로 암 연구에 활용. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | rVU-1D9 |
| Immunogen | Recombinant full-length human EpCAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Dog, Cat, Rat, or Ferret.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a Ca²⁺-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle regulators cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM correlates with enhanced epithelial proliferation and tumor progression.
Ep-CAM is highly expressed in human carcinomas and serves as a marker for epithelial lineage tumors. It is localized on the basolateral surface of most simple epithelia and carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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