Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 660, eBioscience

Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 660, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Phosphorylation of tyrosine residues plays a key role in intracellular signaling cascades. Detection of p-Tyr residues aids in the characterization and purification of phosphorylated proteins and associated signaling pathways.

Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for intracellular staining of normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.

Staining Protocols:

• Protocol A (Two-step, cytoplasmic): For detection of surface and intracellular proteins.
• Protocol B (One-step, nuclear): For transcription factor staining with phosphorylated intracellular proteins.
• Protocol C (Fixation/Methanol): For distinguishing phospho-specific signaling between stimulated and unstimulated samples.

Detailed protocols available in the BestProtocols® section.

Fluorescence Characteristics:
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtered through 0.2 µm post-manufacturing.

Target Information

Tyrosine phosphorylation is central to signal transduction, mitogenesis, and oncogenic transformation. Antibodies specific to phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and related biochemical pathways. The pY20 clone is particularly useful for detecting these proteins in immunohistochemical and immunocytochemical applications where secondary antibodies may complicate detection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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