Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 660, eBioscience
단일클론 항체 pY20은 인산화된 티로신 잔기를 특이적으로 인식하여 세포 신호전달 연구에 유용합니다. eFluor 660 형광 표지로 유세포분석(Flow Cytometry)에 적합하며, 633–647 nm 레이저에서 668 nm 방출을 가집니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eFluor 660, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | pY20 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2574230 |
Additional Formats
Product Specific Information
The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Phosphorylation of tyrosine residues plays a key role in intracellular signaling cascades. Detection of p-Tyr residues aids in the characterization and purification of phosphorylated proteins and associated signaling pathways.
Applications Reported:
This antibody is suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for intracellular staining of normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.
Staining Protocols:
- Protocol A (Two-step, cytoplasmic): For detection of surface and intracellular proteins.
- Protocol B (One-step, nuclear): For transcription factor staining with phosphorylated intracellular proteins.
- Protocol C (Fixation/Methanol): For distinguishing phospho-specific signaling between stimulated and unstimulated samples.
Detailed protocols available in the BestProtocols® section.
Fluorescence Characteristics:
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtered through 0.2 µm post-manufacturing.
Target Information
Tyrosine phosphorylation is central to signal transduction, mitogenesis, and oncogenic transformation. Antibodies specific to phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and related biochemical pathways. The pY20 clone is particularly useful for detecting these proteins in immunohistochemical and immunocytochemical applications where secondary antibodies may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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