Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr458, Tyr199) Recombinant Rabbit Monoclonal Antibody (PI3KY458-1A11)
PI3K p85/p55의 인산화 형태(Tyr458, Tyr199)를 특이적으로 인식하는 recombinant rabbit monoclonal antibody. 높은 특이성과 재현성을 제공하며, Flow Cytometry 등 다양한 응용 가능. HEK293 발현 시스템에서 생산된 동물유래 성분 없는 항체.
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Applications
- Flow Cytometry (Flow): 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PI3KY458-1A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896888 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases via its SH2 domain, acting as an adapter that mediates association of the p110 catalytic unit to the plasma membrane.
Phospho PI3K is essential for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it binds to IRS-1. Growth factors and hormones trigger this phosphorylation, coordinating cell growth, cycle entry, migration, and survival.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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