Thermo Fisher Scientific POLI Polyclonal Antibody
POLI 단백질을 인식하는 Rabbit polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, DNA 복구 및 전사 연구에 활용됩니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human POLI (Accession Q9UNA4), corresponding to amino acid residues M1–N49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851929 |
Product Specific Information
Antibody detects endogenous levels of total POLI.
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, preferentially inserting guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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