Thermo Fisher Scientific ATP6V1A Monoclonal Antibody (GT811)
ATP6V1A 단백질을 검출하기 위한 Thermo Fisher Scientific의 GT811 모노클론 항체. 인간, 생쥐, 랫드 등 다양한 종에 반응하며 WB, ICC/IF, Flow Cytometry에 사용 가능. 고순도 Protein A 정제, 액상 형태로 제공되며 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT811 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1A (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.99 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735124 |
Product Specific Information
- Positive Control: 293T, HepG2
- Predicted Reactivity: Mouse (96%), Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (83%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is necessary for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase is composed of:
- V1 domain (cytosolic): three A and three B subunits, two G subunits, plus C, D, E, F, and H subunits (contains the ATP catalytic site)
- V0 domain (transmembrane): five different subunits (a, c, c’, c’’, and d)
Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.
Transcript variants derived from alternative polyadenylation exist.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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