Thermo Fisher Scientific PHD1 Polyclonal Antibody
PHD1 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, Affinity chromatography로 정제된 액상 형태입니다. 세포 내 산소 감지 및 성장 조절 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EGLN2 (Accession Q96KS0), corresponding to amino acid residues H49–L99 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852325 |
Product Specific Information
Antibody detects endogenous levels of total EGLN2.
Target Information
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2, and HIF PHD3 (known as PHD1, PHD2, and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits.
Hypoxia-inducible factor (HIF) is a transcriptional regulator important in oxygen homeostasis.
The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
HIF PHD1 is widely expressed, with the highest levels in testis, and functions as a cellular oxygen sensor important in cell growth regulation.
It can localize to the nucleus or cytoplasm and is also detected in hormone-responsive tissues such as mammary, ovarian, and prostate epithelium.
HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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