Thermo Fisher Scientific GluD1 Polyclonal Antibody
Thermo Fisher Scientific의 GluD1 Polyclonal Antibody는 인간 Glutamate Dehydrogenase를 인식하는 토끼 IgG 항체로, WB, IHC, ICC 등 다양한 분석에 사용됩니다. 높은 특이성과 안정성을 제공하며 연구용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Glutamate Dehydrogenase. Recombinant protein control fragment (Product #RP-104374). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642056 |
Product Specific Information
Immunogen sequence:
TFVVQGFGNV GLHSMRYLHR FGAKCIAVGE SDGSIWNPDG IDPKELEDFK LQHGSILGFP KAKPYEGSIL
Ortholog sequence identity:
- Mouse: 96%
- Rat: 96%
Target Information
Glutamate dehydrogenase (GluD1) is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. It plays a key role in regulating amino acid-induced insulin secretion and is allosterically activated by ADP while inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form. Related pseudogenes exist on chromosomes 10, 18, and X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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