Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), PE-eFluor 610, eBioscience
인간 CD123(IL-3 수용체 알파체인)을 인식하는 마우스 단클론 항체. PE-eFluor 610으로 결합되어 유세포분석(Flow Cytometry)에 최적화. 488–561 nm 레이저로 여기, 607 nm에서 방출. 4°C 암소 보관, 동결 금지. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | – | View 2 publications |
| Immunohistochemistry (Frozen) (IHC-F) | – | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 8 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574577 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5 / PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702
- Request custom conjugation
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor (60–70 kDa). This transmembrane protein binds IL-3 with low affinity alone and high affinity when associated with CD131 (common beta chain). CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (in 100 µL final volume).
Fluorophore Information:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended detection filter: 610/20 band pass (equivalent to PE-Texas Red®). Protect from light due to photo-induced oxidation sensitivity.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and forms a heterodimer with the common beta (CDw131) subunit. This complex transmits IL-3 signaling. IL-3Ra is overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making it a potential therapeutic target.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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