Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), PE-eFluor 610, eBioscience

Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test
• Applications Reported: Flow cytometric analysis, intracellular staining followed by flow cytometric analysis
• Applications Tested: Intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using FoxP3/Transcription Factor Staining Buffer Set (Product #00-5523-00)

Publications

• View 16 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 737
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The UC10-4B9 monoclonal antibody reacts with mouse CD152 (CTLA-4), a protein structurally similar to CD28. CTLA-4 is expressed on activated T cells and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28, delivering inhibitory signals that restrict T-cell activation by reducing IL-2 secretion and proliferation.

Due to its intracellular localization, detection may require assessment of both surface and intracellular expression.

Experimental Notes

• Tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes.
• Recommended use ≤ 0.5 µg/test (100 µL final volume).
• Adjust cell number empirically (10⁵–10⁸ cells/test).
• Carefully titrate antibody for optimal performance.

Fluorophore Information

• Excitation: 488–561 nm
• Emission: 607 nm
• Recommended Filter: 610/20 band pass (PE-Texas Red® equivalent)
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Light Sensitivity: Protect from light (photo-induced oxidation risk)

Fixation

Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Filtration

• 0.2 µm post-manufacturing filtered.

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4+ T cells. It binds CD80 and CD86 with higher affinity than CD28, delivering inhibitory signals that suppress T-cell activation. Intracellular CTLA4 is also found in regulatory T cells and contributes to their suppressive function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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