Thermo Fisher Scientific RNA Polymerase II Polyclonal Antibody
Rabbit polyclonal antibody targeting the C-terminal repeat of human RNA Polymerase II. Validated for WB and IP applications. Supplied as an unconjugated liquid at 0.2 mg/mL. For research use only; store at 4°C and ship on wet ice.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Category | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C-terminal repeat of Human RNA Polymerase II |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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