Applied Biosystems™

CLA GlobalFiler™ PCR Amplification Kit

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines. GlobalFiler PCR자세히 알아보기

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines. GlobalFiler PCR amplification kits are 6-dye, 24-locus short tandem repeat (STR) analysis kits that combine maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, enabling labs with improved overall efficiency. GlobalFiler kits are backed by best-in-class global training, service, and support. This kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.

Features of the CLA GlobalFiler PCR Amplification Kit include:

• 6-dye, 24-locus STR analysis kit compatible with all cell line databasing loci standards
• Maximum compatibility with global databasing loci standards
• Reduced amplification time and superior discrimination power
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, 3500/xL and SeqStudio Flex (8- and 24-capillary) genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

사양

검출 방법Gel Electrophoresis

형태Liquid

형식Kit

GC-Rich PCR PerformanceHigh

PCR 방법End-point PCR

중합효소AmpliTaq Gold™ DNA Polymerase

반응 속도60 min.

기술STR (Short Tandem Repeats) Analysis

용도(장비)SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers

키트 유형Multicomponent

라벨 또는 염료LIZ, FAM, NED, PET, VIC

수량200 x 25 μL Reactions

종Human

타겟 유기체 분류Human cells

용도(애플리케이션)Genotyping, Cell Analysis

정제 타겟Genomic DNA

반응 형식Master Mix

샘플 종류Genomic DNA

시작 물질Cell Line Culture

Unit SizeEach