Thermo Fisher Scientific XPF Monoclonal Antibody (UMAB21), UltraMAB
XPF 단백질을 인식하는 UMAB21 클론의 Mouse IgG1 단일클론 항체. Western blot, IHC, ICC, ChIP, Peptide Array 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며 연구용으로 적합.
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- UM500021CF
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | Assay-Dependent |
| Peptide Array (Array) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB21 |
| Immunogen | Full length human recombinant protein of human XPF produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The ERCC1/XPF endonuclease complex is involved in repairing two types of DNA lesions:
- Nucleotide excision repair (NER) for UV-induced lesions and bulky chemical adducts
- Recombination repair of interstrand cross-links
In mammalian cells, XPG cleaves 3′ of the DNA lesion while ERCC1/XPF performs the 5′ incision. This complex likely also processes partially repaired recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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