Thermo Fisher Scientific IDH1 Monoclonal Antibody (OTI24A2), TrueMAB
IDH1 (R132H) 변이 단백질을 인식하는 단클론 항체로, Western blot 및 IHC(Paraffin) 검증 완료. Mouse IgG2b 아이소타입, 동결건조 형태로 제공. 연구용으로 세포 및 조직 내 IDH1 변이 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI24A2 |
| Immunogen | Synthetic peptide around the R132 region of the human IDH conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Isocitrate dehydrogenases (IDH1) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes are divided into NAD(+)-dependent and NADP(+)-dependent subclasses.
The IDH1 enzyme encoded by this gene is NADP(+)-dependent and localized in the cytoplasm and peroxisomes. It plays a crucial role in NADPH regeneration for peroxisomal and cytoplasmic metabolic reactions.
This antibody specifically recognizes the IDH1 mutant R132H protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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