Thermo Fisher Scientific CD45 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CD45 for IHC(P) applications. High specificity and purity via antigen affinity chromatography. Suitable for hematopoietic cell marker studies. Supplied in liquid form with 1 mg/mL concentration, stored at 4°C ...
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Applications and Tested Dilution
- Application: Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 2.5–5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 16 amino acid peptide from internal region of human CD45 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550469 |
Product Specific Information
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (88%)
- For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
- After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase–streptavidin and chromogen.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, comprising about 10% of all surface proteins in lymphocytes. It is absent in non-hematopoietic cells and tissues.
CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, regulating Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions.
CD45RA, one isoform, shows restricted expression among lymphoid subtypes. Isoform expression depends on cell type and physiological state, influencing membrane localization and signaling.
CD45 regulates T- and B-cell receptor signaling, modulates JAK kinase activity, and participates in integrin-mediated survival signaling, apoptosis regulation, and immune function modulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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