Thermo Fisher Scientific CALM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 420 and 646 of Human CALM if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CALM,uniProtId:Q13492-1,ncbiNodeId:9606,antigenRange:420-646,antigenLength:652,antigenImageFileName:PA5-31520_CALM_Q13492-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31520_CALM_Q13492-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548993

Product Specific Information

Recommended positive controls: A549, HeLa, HepG2, NIH-3T3.

Predicted reactivity: Mouse (95%), Rat (86%), Pig (98%), Rhesus Monkey (93%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer`s disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.