Thermo Fisher Scientific ISG15 Polyclonal Antibody
ISG15 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등에 사용 가능. 인간 및 랫트 반응성. 고순도 Affinity Chromatography 정제, -20°C 보관. 세포 내 위치는 Cytoplasm.
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Thermo Fisher Scientific ISG15 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | View publication |
| ELISA | 1 µg/mL | – |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–165 of human ISG15 (NP_0050921) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.74 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804784 |
Product Specific Information
Immunogen sequence:
MGWDLTVKML AGNEFQVSLS SSMSVSELKA QITQKIGVHA FQQRLAVHPS GVALQDRVPL ASQGLGPGST VLLVVDKCDE PLSILVRNNK GRSSTYEVRL TQTVAHLKQQ VSGLEGVQDD LFWLTFEGKP LEDQLPLGEY GLKPLSTVFM NLRLRGGGTE PGGRS
Positive Samples: HeLa, PC-3, SW620, HCT116
Cellular Location: Cytoplasm
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to target proteins (ISGylation) or as a free protein. It is secreted from monocytes in response to type I interferons and enhances NK-cell proliferation and cytotoxicity. ISG15 synthesis is stimulated by IFN-α, IFN-β, or IFN-ω, but not IFN-γ. It is induced by overexpression of interferon regulatory factors and by influenza B virus. ISG15 is secreted by various cell lines including monocytes, T-lymphocytes, B-lymphocytes, fibroblasts, and epithelial cells. Mutations in the ISG15 gene can cause Immunodeficiency 38 with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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