Thermo Fisher Scientific NONO Monoclonal Antibody (OTI3F8), TrueMAB
NONO 단백질을 인식하는 Mouse monoclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 인간, 개, 생쥐 반응성. Lyophilized 형태, PBS buffer에 8% trehalose 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 184–385 of human NONO produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NONO gene encodes an RNA-binding protein involved in various nuclear processes, including transcriptional regulation and RNA splicing.
A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.
Alternatively spliced transcript variants have been described, and pseudogenes exist on Chromosomes 2 and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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