Thermo Fisher Scientific CD83 Recombinant Rabbit Monoclonal Antibody (005)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.

Advantages include:

• High specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broad immunoreactivity due to the rabbit immune repertoire

This antibody has specificity for Mouse CD83.

Target Information

CD83 is a 40–45 kDa cell surface glycoprotein expressed on peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 when stimulated with agents such as Con A or PHA.

In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by cells within germinal centers. It is also expressed by Langerhans cells in the skin.

CD83 is a 186-amino-acid single-chain glycoprotein, a member of the immunoglobulin superfamily, composed of:

• An extracellular V-type Ig-like domain
• A transmembrane region
• A short cytoplasmic tail (~40 amino acids)

CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of its core protein. CD83⁺ cells have a unique immunophenotype distinct from T, B, NK, or myelomonocytic cells, and co-express high levels of MHC class II molecules, along with markers such as CD2, CD5, CD40, CD78, CD13, CD33, and CD36.

Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.