Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet 661, eBioscience
Flow cytometry용 Rat IgG2a kappa 동형대조 항체로, Brilliant Ultra Violet 661 형광체 결합. 실험 항체의 비특이적 결합 평가에 사용되며, UV(355 nm) 레이저 장착 장비에 적합. 4°C 암소 보관, 동결 금지.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | Brilliant Ultra Violet™ 661 |
| Excitation/Emission Max | 349 / 659 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2921011 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.
Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells; use at the same concentration as the experimental antibody.
Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye emitting at 660 nm, intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.
Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Rat IgG2a is an immunoglobulin subclass produced by the rat immune system, known for complement activation and antibody-dependent cell-mediated cytotoxicity (ADCC).
It has a molecular weight of approximately 160 kDa, composed of two heavy and two light chains.
Commonly used as a control or reference antibody in experiments involving rat models, flow cytometry, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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