Thermo Fisher Scientific CISD2 Polyclonal Antibody
인간 CISD2 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol 완충액에 보관. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CISD2. Recombinant protein control fragment (Product #RP-90311). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2639825 |
Product Specific Information
Immunogen sequence:
PKKKQQKDSL INLKIQKENP KVVNEINIED LCLTKAAYCR CWRSKTFPAC DGSHNKHNEL TGDNVGPLIL KKKEV
Sequence identity:
- Mouse: 100%
- Rat: 97%
Target Information
Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. It interacts with Bcl-2 and can be displaced by the BH3-only protein BIK, contributing to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death and premature aging phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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