Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70.15), FITC
마우스 CD11b 단백질을 표적하는 Rat IgG2b 단클론 항체로, FITC 형광 표지되어 흐름세포분석(FACS)에 적합합니다. 단백질 G로 정제되었으며, PBS/BSA 버퍼에 보존됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70.15), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.2 µg/1x10^6 cells | View 7 publications |
| Miscellaneous PubMed (Misc) | - | View 15 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70.15 |
| Immunogen | C57BL/6 spleen cell enriched for T lymphocytes |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539243 |
Additional Formats
Product Specific Information
MA5-17859 targets CD11b/ITGAM/Integrin alpha M in FACS applications and shows reactivity with mouse samples.
The immunogen is C57BL/6 spleen cell enriched for T lymphocytes.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex, also known as Mac-1 or CR3, serves as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
CD11b is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cell subsets, dendritic cells, and microglia. It is commonly used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies by tissue macrophage type.
Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune disorders.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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