Thermo Fisher Scientific CD80 Monoclonal Antibody (UMAB65), UltraMAB
Human CD80 단백질을 인식하는 Mouse IgG2b 단클론 항체로, IHC(P), ChIP, Peptide Array 등에 사용 가능. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼, -20°C 보관. 연구용으로 T 세포 활성화 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| ChIP assay (ChIP) | Assay-Dependent | - |
| Peptide Array (Array) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB65 |
| Immunogen | Full length human recombinant protein of human CD80 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152).
Both are expressed on antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes.
Dysfunction of CD80 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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