Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 436, eBioscience
마우스 Sca-1(Ly-6A/E) 항원을 인식하는 D7 단클론 항체로, Super Bright 436 형광체로 결합됨. Flow cytometry에 최적화되어 있으며, 405 nm 보라색 레이저로 여기되어 436 nm에서 방출. 마우스 조혈모세포 및 림프구 표지에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 6 publications |
| Immunocytochemistry (ICC/IF) | – | View 5 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 85 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | D7 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2637286 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395, 661, 737, 805
- Brilliant Violet 421, 480, 605, 711, 786
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 600, Super Bright 645
- Request custom conjugation
Product Specific Information
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 binds both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains (C57BL, SJL, 129, AKR, etc.) show higher D7-positive cell frequency compared to Ly-6.1 strains (BALB/c, C3H, NZB, etc.). Sca-1 expression increases upon activation regardless of haplotype.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometry on mouse bone marrow cells, ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Super Bright 436 is excited with the violet laser line (405 nm) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Stem Cell Antigen-1 (Sca-1) is a member of the Ly-6 family, expressed on multipotent hematopoietic stem cells (HSC) and used as a marker for murine HSC. It is co-used with lineage depletion antibodies for HSC identification. Sca-1 is found in fetal liver, adult bone marrow, peripheral blood, and spleen, and may regulate B and T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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