Thermo Fisher Scientific CD62E (E-selectin) Polyclonal Antibody
Thermo Fisher Scientific의 CD62E (E-selectin) Polyclonal Antibody는 인간 시료에 반응하는 토끼 IgG 항체로, Western blot, ICC/IF, Flow Cytometry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 염증 관련 내피세포 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human CD62E (aa 26–75/610) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932629 |
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion also occurs in capillaries and small venules throughout the body after the onset of inflammation. This process involves a cascade of adhesion receptors, including members of the selectin and immunoglobulin superfamilies.
E-selectin (CD62E) is an endothelial cell-specific C-type lectin expressed only after activation by proinflammatory cytokines such as IL-1, TNFα, and bacterial components like lipopolysaccharides. Its expression is regulated through an NFκB-dependent signaling cascade and is associated with vascular endothelium in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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