Thermo Fisher Scientific eIF3a Polyclonal Antibody
Western blot, ICC/IF, IP에 사용 가능한 eIF3a 폴리클로날 항체. 인간, 마우스, 랫드, 영장류에 반응. 항원 친화 크로마토그래피로 정제된 Rabbit IgG. 단백질 번역 개시 인자 eIF3 복합체 연구에 적합. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | View 1 publication |
| Immunoprecipitation (IP) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a sequence of human eIF3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 15.2 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984921 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773).
The eIF-3 complex associates with the 40S ribosome and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S pre-initiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732).
It specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation, and apoptosis, using different RNA stem-loop binding modes to exert translational activation or repression (PubMed:25849773, PubMed:27462815).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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