Thermo Fisher Scientific beta Actin Polyclonal Antibody
다양한 종에서 교차 반응성을 보이는 β-Actin 폴리클로날 항체. Western blot, IHC, ICC, Flow cytometry 등 다중 응용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체. 세포 구조 및 단백질 발현 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:2,000 | 21 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
| Flow Cytometry (Flow) | 1:10–1:1,000 | - |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| Neutralization (Neu) | Assay-Dependent | 1 publication |
| in situ PLA (PLA) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Avian, Bovine, Fish, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Published Species | Bovine, Human, Mouse, Pig, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the N-terminus of Human beta-Actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/tris phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2223196 |
Product Specific Information
This antibody is expected to cross-react with a wide range of species based on sequence homology.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types, involved in cell motility, structure, and integrity.
Beta and gamma actins coexist in most cells as cytoskeletal components. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin), a two-stranded helix structure.
Due to its ubiquitous expression, beta actin is widely used as a loading control in protein assays such as Western blotting.
Antibodies to beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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