Thermo Fisher Scientific Rat anti-Mouse IgE (Heavy chain) Secondary Antibody, FITC

Applications

Tested Dilution

Flow Cytometry (Flow)

5 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

LO-ME-3

Immunogen

Purified IgE from BALB/c mice

Conjugate

FITC FITC FITC

View additional formats

• [Biotin ](/antibody/secondary/product-name/Mouse IgE (Heavy chain)/conjugates/Biotin/host/Rat/)
• [HRP](/antibody/secondary/product-name/Mouse IgE (Heavy chain)/conjugates/HRP/host/Rat/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538263

Target

IgE (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

This antibody does not react with rat.

For FACS analysis, use 50 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.