Thermo Fisher Scientific Rat anti-Mouse IgE (Heavy chain) Secondary Antibody, FITC

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µg/mL

Product Specifications

Additional Formats

• Biotin conjugate
• HRP conjugate
• Custom conjugation available upon request

Product Specific Information

• This antibody does not react with rat.
• For FACS analysis, use 50 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
• Rat anti-Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain and does not cross-react with other murine immunoglobulin classes or subclasses.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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