Thermo Fisher Scientific SARS-CoV-2 Nucleocapsid (aa1-419), His Tag Recombinant Protein
SARS-CoV-2 Nucleocapsid 단백질(aa1-419)은 E. coli에서 발현된 His-tag 재조합 단백질로, ELISA 및 Western blot 등 다양한 분석에 활용 가능. >95% 고순도, 낮은 엔도톡신 수준, 바이러스 진단 연구용으로 적합. -80°C에서 안정적으로 보관.
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- RP87707
- 카테고리
- Protein
- 판매단위
- pk
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Applications
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | E. coli |
| Amino Acid Sequence | SARS-CoV-2 Nucleocapsid, Met1-Ala419 with an N-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 49.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE; >95% by HPLC |
| Endotoxin Concentration | <1.0 EU/µg |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.56 mg/mL |
| Purification | Purified |
| Storage Buffer | 20 mM Tris, pH 8.0, with 0.1 mM EDTA, 500 mM NaCl, 10% glycerol |
| Contains | No preservative |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 µg/mL (100 µL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12–1.64 ng/mL.
Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 µg/mL (100 µL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.
Product Specific Information
- Animal Origin Free
- Storage: Store the lyophilized protein at -20°C to -80°C for long term.
- Stable at -70°C for up to 1 year from the date of receipt.
Target Information
SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid and is the most abundant coronavirus protein.
Due to its strong immunogenicity, it has potential diagnostic value for SARS-CoV-2.
The N protein is an RNA-binding protein critical for viral genome packaging, containing dynamic disordered regions with transiently-helical motifs.
It acts as a flexible and multivalent RNA-binding protein, contributing to viral replication and assembly.
SARS-CoV-2 causes illnesses ranging from the common cold to severe diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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