Thermo Fisher Scientific Mouse VEGF-165, Animal-Free Recombinant Protein, PeproTech
Mouse VEGF-165 재조합 단백질로, E. coli 발현 시스템에서 생산된 동물 유래 성분 없는 고순도 단백질입니다. 인간 제대정맥 내피세포(HUVEC) 증식 활성 검증 완료. ELISA 및 기능적 분석에 적합하며, ≥98% 순도와 낮은 엔도톡신 함량을 보유합니다.
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Applications
ELISA (ELISA)
- Tested Dilution: Not specified
- View 1 publication
Functional Assay (Functional)
- Assay-dependent
- View publication
In vitro Assay (IV)
- Tested Dilution: Not specified
- View 3 publications
Miscellaneous PubMed (Misc)
- Tested Dilution: Not specified
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Published Species | Amphibian, Mouse |
| Expression System | E. coli |
| Amino Acid Sequence | MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Molecular Weight | 39 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | <0.1 EU/µg |
| Activity | Dose-dependent stimulation of HUVEC proliferation (1.0–5.0 ng/ml) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Murine VEGF-165 is a 39.0 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF and its receptor system are fundamental regulators of angiogenesis signaling. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine associated with this process.
Five different isoforms of VEGF are generated through alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which are expressed mainly on endothelial cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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