Thermo Fisher Scientific TRIM21 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 86-334 of human TRIM21/SS-A (NP_0031322) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM21,uniProtId:P19474-1,ncbiNodeId:9606,antigenRange:86-334,antigenLength:475,antigenImageFileName:PA5-120224_TRIM21_P19474-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120224_TRIM21_P19474-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.71 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913796

Product Specific Information

Positive test controls include: A-549, Jurkat, HeLa. The target is usually found in the following locations: Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, autophagosome.

Immunogen sequence: GTQGERCAVH GERLHLFCEK DGKALCWVCA QSRKHRDHAM VPLEEAAQEY QEKLQVALGE LRRKQELAEK LEVEIAIKRA DWKKTVETQK SRIHAEFVQQ KNFLVEEEQR QLQELEKDER EQLRILGEKE AKLAQQSQAL QELISELDRR CHSSALELLQ EVIIVLERSE SWNLKDLDIT SPELRSVCHV PGLKKMLRTC AVHITLDPDT ANPWLILSED RRQVRLGDTQ QSIPGNEERF DSYPMVLGA

Target Information

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.