Thermo Fisher Scientific TRIM21 Polyclonal Antibody
TRIM21 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. PBS/glycerol buffer에 보관되며 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 86–334 of human TRIM21/SS-A (NP_0031322) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.71 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913796 |
Product Specific Information
- Positive test controls: A-549, Jurkat, HeLa
- Subcellular localization: Cytoplasm, Cytoplasmic vesicle, Nucleus, P-body, Autophagosome
Immunogen sequence:
GTQGERCAVH GERLHLFCEK DGKALCWVCA QSRKHRDHAM VPLEEAAQEY QEKLQVALGE LRRKQELAEK LEVEIAIKRA DWKKTVETQK SRIHAEFVQQ KNFLVEEEQR QLQELEKDER EQLRILGEKE AKLAQQSQAL QELISELDRR CHSSALELLQ EVIIVLERSE SWNLKDLDIT SPELRSVCHV PGLKKMLRTC AVHITLDPDT ANPWLILSED RRQVRLGDTQ QSIPGNEERF DSYPMVLGA
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains (a RING, a B-box type 1, and a B-box type 2) and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus and interacts with autoantigens in patients with Sjögren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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