Thermo Fisher Scientific HMCES Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 40 µL/mg of lysate

Product Specifications

Product Specific Information

Predicted reactivity by homology: Orangutan.

Target Information

Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877, PubMed:31235915, PubMed:31235913).
Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA, forming a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235913).
The HMCES DNA-protein cross-link is degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a "suicide" enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877).
Has preference for ssDNA but can also accommodate double-stranded DNA with 3′ or 5′ overhang (dsDNA), and dsDNA-ssDNA 3′ junction (PubMed:31235915, PubMed:31806351).
Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway.
Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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