Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Cyclin B1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합. 인간 시료 반응성, 항원 친화 크로마토그래피 정제, 고농도(0.8 mg/mL) 액상 형태. 세포주기 G2/M 전이 조절 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CCNB1. Recombinant protein control fragment (Product #RP-105188). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791650 |
Product Specific Information
Immunogen sequence:
TRNSKINAEN KAKINMAGAK RVPTAPAATS KPGLRPRTAL GDIGNKVSEQ LQAKMPMKKE AKPSATGKVI DK
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts exist: a constitutively expressed transcript and a cell cycle-regulated transcript expressed predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is destroyed at the end of mitosis via APC-dependent degradation.
Cyclin B1 overexpression has been observed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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