Thermo Fisher Scientific SMAD5 Recombinant Rabbit Monoclonal Antibody (ARC0448)
SMAD5 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, ELISA, IP에 사용 가능. 인간 및 생쥐 반응성. 고순도 Affinity Chromatography 정제, 액상 형태로 제공. 연구용 전용 제품.
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Thermo Fisher Scientific SMAD5 Recombinant Rabbit Monoclonal Antibody (ARC0448)
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:1,000
- ELISA: 1 µg/mL
- Immunoprecipitation (IP): 0.5 µg–4 µg antibody for 200 µg–400 µg extracts
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0448 |
| Immunogen | Synthetic peptide corresponding to amino acids 200–300 of human Smad5 (Q99717) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849172 |
Product Specific Information
Immunogen sequence:
STYPNSPASS GPGSPFQLPA DTPPPAYMPP DDQMGQDNSQ PMDTSNNMIP QIMPSISSRD VQPVAYEEPK HWCSIVYYEL NNRVGEAFHA SSTSVLVDGF T
Target Information
SMADs are members of the MAD-related family of molecules. These intracellular proteins are key components in signaling pathways of the serine/threonine kinase receptors of the TGF-beta superfamily.
SMADs are categorized as:
- R-SMADs: SMAD1, 2, 5, 8, 9
- Co-SMAD: SMAD4
- I-SMADs: SMAD6, 7
SMAD1, 5, 8, and 9 share high homology, and antibodies recognizing common sequences are available. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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