Thermo Fisher Scientific WIPI2 Polyclonal Antibody
Human WIPI2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. PBS/glycerol 완충액에 보관하며 연구용으로 적합.
- 카탈로그번호
- PA554425
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human WIPI2. Recombinant protein control fragment (Product #RP-92774). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2649655 |
Product Specific Information
Immunogen sequence:
MKVLHTIRET PPNPAGLCAL SINNDNCYLA YPGSATIGEV QVFDTINLRA ANMIPAHDSP LAALAFDASG TKLATASEKG TVIRVF
Highest antigen sequence identity to the following orthologs:
- Mouse: 99%
- Rat: 100%
Target Information
WD repeat proteins regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions.
WIPI2 (also known as ATG18B or ATG21) is a human homolog to yeast ATG18 and contains three WD repeats forming a 7-bladed propeller structure.
It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes.
Together with WIPI1, WIPI2 is found at the plasma membrane and autophagosomal membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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