Thermo Fisher Scientific AKAP3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (TPLQLLDWLMVNL) corresponding to the C-terminus amino acids of AKAP3 (aa 841-853). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKAP3,uniProtId:O75969-1,ncbiNodeId:9606,antigenRange:841-853,antigenLength:853,antigenImageFileName:PA5-18261_AKAP3_O75969-1_Goat.svg,antigenImageFileNamePDP:PA5-18261_AKAP3_O75969-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10975501

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP). AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA. AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.