Thermo Fisher Scientific ZBED1 Monoclonal Antibody (OTI1A1), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ZBED1 (also known as DREF) is the human homolog of the Drosophila transcriptional regulatory factor DREF, which is required for expression of genes involved in DNA replication and cell proliferation. RNA interference experiments targeting ZBED1 RNA inhibit S phase entry and reduce histone H1 mRNA in HeLa cells, indicating its role in cell proliferation. At least 22 ribosomal proteins have ZBED1 binding sites in their promoters; anti-ZBED1 RNAi decreases RP gene expression. Immunoprecipitation analyses show that ZBED1 self-associates via a carboxyl-terminal hATC domain, essential for nuclear localization and DNA binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.