Thermo Fisher Scientific OCT4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Product Specifications

Species Reactivity

Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 107-132 amino acids from the Central region of Zebrafish pou5f1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2718129

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.