Thermo Fisher Scientific IgD Monoclonal Antibody (11-26c (11-26)), APC-eFluor 780, eBioscience
마우스 IgD 델타 중쇄를 인식하는 Rat IgG2a 단일클론 항체로, APC-eFluor 780 형광체 결합. 유세포 분석용으로 최적화되어 있으며 0.125 µg/test 농도로 사용 가능. 광민감성 주의, 4°C 암소 보관.
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Thermo Fisher Scientific IgD Monoclonal Antibody (11-26c (11-26)), APC-eFluor 780, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11-26c (11-26) |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573994 |
Additional Formats
- Alexa Fluor 700
- APC
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG, or IgM. IgD is expressed by peripheral mature B cells, and 11-26c does not activate B cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration recommended for optimal assay performance.
Fluorophore Information:
APC-eFluor® 780 emits at 780 nm and is excited by the Red laser (633 nm). Ensure instrument compatibility.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark. Clone-specific fixation performance should be determined empirically.
Excitation / Emission / Laser:
633–647 nm / 780 nm / Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IgD is a type I transmembrane protein co-expressed on mature naive B cells with membrane IgM. It also exists in soluble form in serum and participates in B cell receptor-mediated signal transduction upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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