Thermo Fisher Scientific FBXW7 Monoclonal Antibody (OTI13H4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

F-Box and WD Repeat Domain Containing 7 (FBXW7) is a member of the F-box protein family, characterized by a ~40 amino acid F-box motif.
These proteins form part of the SCF (SKP1–Cullin–F-box) ubiquitin ligase complex, functioning in phosphorylation-dependent ubiquitination.
F-box proteins are classified into three groups:

• Fbws: Contain WD-40 domains
• Fbls: Contain leucine-rich repeats
• Fbxs: Contain other interaction modules or no recognizable motifs

FBXW7, also known as FBX30, belongs to the Fbws class and includes seven tandem WD40 repeats. It binds directly to cyclin E, likely targeting it for ubiquitin-mediated degradation.
Mutations in FBXW7 have been observed in ovarian and breast cancer cell lines, suggesting a role in cancer pathogenesis.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.