Thermo Fisher Scientific CFLAR Monoclonal Antibody (2F12)
CFLAR 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 2F12). Western blot, IHC, ICC, ELISA에 적합하며, 인간 시료 반응성. PBS 버퍼 내 액상 형태로 제공되며, -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F12 |
| Immunogen | CFLAR (AAH01602, 178 a.a. approximately 277 a.a) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
AGTSYRNVLQ AAIQKSLKDP SNNFRLHNGR SKEQRLKEQL GAQQEPVKKS IQESEAFLPQ SIPEERYKMK SKPLGICLII DCIGNETELL RDTFTSLGYE
Target Information
FLIP (CFLAR) is an apoptosis regulator protein that functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6-mediated apoptosis. A proteolytic fragment (p43) is retained in the death-inducing signaling complex (DISC), blocking further recruitment and processing of caspase-8. Full-length and shorter isoforms may either induce or reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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