Thermo Fisher Scientific CFLAR Monoclonal Antibody (2F12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.3 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F12

Immunogen

CFLAR (AAH01602, 178 a.a. approximately 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FLIP,uniProtId:O15519-1,ncbiNodeId:9606,antigenRange:178-277,antigenLength:480,antigenImageFileName:H00008837-M01_FLIP_O15519-1_House_mouse.svg,antigenImageFileNamePDP:H00008837-M01_FLIP_O15519-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: AGTSYRNVLQ AAIQKSLKDP SNNFRLHNGR SKEQRLKEQL GAQQEPVKKS IQESEAFLPQ SIPEERYKMK SKPLGICLII DCIGNETELL RDTFTSLGYE

Target Information

FLIP (CFLAR) is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.