Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human HMGB1.

Target Information

HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding chromosomal protein. It can be released by damaged or necrotic cells into the extracellular environment, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215-amino acid single-chain polypeptide with three domains:

• Two positively charged DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail

Residues 28–44 and 180–185 contain nuclear localization signals (NLS). The cytokine activity resides in the B box, while the A box associates with transcription factors’ helix-loop-helix domains.

HMGB1 acts as an inflammatory mediator promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interactions. It signals via receptors such as RAGE and possibly TLR2 and TLR4.

Highly conserved and ubiquitously expressed in nuclei and cytoplasm, HMGB1 is a key mediator of inflammation in both sterile and infection-associated responses. Elevated HMGB1 levels are observed during tissue injury, infection, endotoxemia, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.