Thermo Fisher Scientific IRF9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 171 and 393 of Human IRF9 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRF9,uniProtId:Q00978-1,ncbiNodeId:9606,antigenRange:171-393,antigenLength:393,antigenImageFileName:PA5-30378_IRF9_Q00978-1_Rabbit.svg,antigenImageFileNamePDP:PA5-30378_IRF9_Q00978-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.63 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547852

Product Specific Information

Recommended positive controls: A549, HeLa, HepG2, HCT116.

Predicted reactivity: Mouse (81%), Pig (87%), Rhesus Monkey (96%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.