Thermo Fisher Scientific SMAD4 Monoclonal Antibody (SMAD4, 2524)

Applications

• ELISA (Direct ELISA)
  • Tested Dilution: Assay-dependent
  • For coating use: 1–5 µg/mL
  • This antibody has only been tested in direct ELISA.

Product Specifications

Product Specific Information

• Antibody tested in direct ELISA only.
• Recommended coating concentration: 1–5 µg/mL.
• For Research Use Only. Not for use in diagnostic procedures.
• Not for resale without express authorization.

Target Information

Smad4 is encoded by the SMAD4 gene located on chromosome 18 in humans. Also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic homolog 4.
Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) domains involved in DNA binding and protein interactions.
Activation of TGF-β or BMP receptors leads to phosphorylation of R-Smads (Smad1, 2, 3, 5, 8), which then form complexes with Smad4 (co-Smad) and translocate to the nucleus to regulate gene expression.
Smad4 functions as a tumor suppressor by regulating genes such as Cyclin D1 (downregulation) and collagen (upregulation), influencing cell proliferation and differentiation.
Dephosphorylation controls nuclear export and nucleocytoplasmic dynamics of Smads.

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