Thermo Fisher Scientific Acid Phosphatase Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross-reactivity against Acid Phosphatase from other tissues and species may occur but has not been specifically determined.

Target Information

Low molecular weight protein tyrosine phosphatases, including the human red cell phosphatase HCPTP, are widely expressed cytosolic proteins (~18 kDa) that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphothreonine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate and has a catalytic mechanism involving the formation of a cystinylphosphate intermediate. The crystal structure of the bovine heart-derived enzyme BHPTP shows a four-stranded central parallel β-sheet with flanking α-helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context (CXXXXXR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.