Thermo Fisher Scientific NEIL3 Polyclonal Antibody
NEIL3 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot 및 면역세포염색(ICC/IF)에 적합하며, 인간 및 생쥐 시료에 반응. PBS/glycerol buffer에 보관되며, DNA 복구 연구용으로 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NEIL3 (Accession Q8TAT5), corresponding to amino acid residues L516–P536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851014 |
Product Specific Information
Antibody detects endogenous levels of total NEIL3.
Target Information
DNA glycosylase which prefers single-stranded DNA (ssDNA) or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg, and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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