Thermo Fisher Scientific CD73 (Immuno-Oncology Target) Monoclonal Antibody (NT5E, 2646)
CD73(NT5E) 단백질을 표적으로 하는 마우스 단클론 항체로, 면역항암 연구용으로 적합. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. Protein A/G 정제 및 PBS 버퍼에 보존. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NT5E, 2646 |
| Immunogen | Recombinant full-length human NT5E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed), incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
This enzyme forms a dimer of two identical 70 kDa subunits attached to the plasma membrane via a glycosyl phosphatidylinositol linkage.
CD73 serves as a marker of lymphocyte differentiation, and its deficiency is associated with various immunodeficiency diseases.
Other 5'-nucleotidase forms exist in the cytoplasm and lysosomes, differing from CD73 in substrate affinity, magnesium ion requirement, ATP activation, and phosphate inhibition.
The CD73 gene is located on chromosome 6q14–q21. Defects can cause joint and arterial calcification and intestinal tuberculosis. Two transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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