Thermo Fisher Scientific DDX60 Polyclonal Antibody
인간 DDX60 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS(glycerol 포함) 용액 형태로 제공. 바이러스 감염 후 발현 증가 및 RIG-I 경로 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DDX60. Recombinant protein control fragment (Product # RP-96654) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2640487 |
Product Specific Information
Immunogen sequence:
PKADKEAHVM ANKLRKVKKS IEKQKIIDEK SQKKTRNVDQ SLIHEAEHDN LVKCLEKNLE IPQDCTYADQ KAVDTETLQK VFGRV
Highest antigen sequence identity to orthologs:
- Mouse: 67%
- Rat: 73%
Target Information
DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of the RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection but is dispensable for TLR3-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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