Thermo Fisher Scientific FOXO1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FOXO1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXO1,uniProtId:Q12778-1,ncbiNodeId:9606,antigenRange:655,antigenLength:655,antigenImageFileName:PA5-85640_FOXO1_Q12778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85640_FOXO1_Q12778-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792780

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Chicken (90%).

Positive Control: Mouse testis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5-TT\[G/A\]TTTTG-3 and the related Daf-16 family binding element (DBE) with consensus sequence 5- TT\[G/A\]TTTAC-3. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.