Thermo Fisher Scientific Collagen IV Polyclonal Antibody
Rabbit polyclonal antibody recognizing Collagen IV alpha 2 for WB, IHC, and ICC applications. Validated for human, mouse, and rat. Supplied as 1 mg/mL liquid in PBS with 50% glycerol. Suitable for detecting endogenous Collagen IV in basement membrane s...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human COL4A2 (Accession P08572), corresponding to amino acid residues F155–G205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899672 |
Product Specific Information
This antibody detects endogenous levels of total Collagen IV alpha 2.
Target Information
Collagen IV is a major structural component of the basement membrane in tissues such as the kidneys, lungs, and skin. It forms a network-like scaffold providing mechanical support and stability. Collagen IV consists of three alpha chains encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes are associated with disorders including Alport syndrome and Goodpasture syndrome, affecting renal and pulmonary function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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